New research presented at the virtual European Respiratory Society International Congress has found that having fewer types of fungi in the lungs is linked to increased severity of disease in acute respiratory distress syndrome (ARDS) patients.
The human microbiome is made up of tiny organisms such as bacteria, viruses and fungi. Presenting her research to the meeting, Noel Britton, a PhD candidate at the University of Pittsburgh, said that up to now researchers had tended to concentrate on studying the effects of bacteria on human health, partly because the microbiome is primarily composed of bacteria and there are technical difficulties in extracting enough fungi to study.
“In terms of numbers of cells, fungi are outnumbered by bacteria by several orders of magnitude,” she said. “Additionally, while it is now well established that the lungs play host to a distinct and dynamic microbiome, they don’t represent a rich environment for microbes to thrive, and they generally have many fewer microbes than the gut–the body site where scientists have focused the most microbiome research. It can be difficult to pick up a signal from such a low mass of fungi and to be sure that the sequences identified are not due to contamination in the laboratory.
“We wanted to carry out this research as acute respiratory distress syndrome is characterized by a hyper-inflammatory over-reaction of the immune system and we know that fungi can be involved in activating and regulating the human immune system. There are no known therapies for the successful treatment of ARDS and very little is known about why some patients have a hyper-inflammatory response. The diversity of the microbiome, and specifically of fungi, may play an important role in understanding why some patients develop ARDS and some do not.”
Britton and her colleagues enrolled 202 mechanically-ventilated patients in the study between October 2011 and September 2019. As far as they know, none of these patients had COVID-19 but they are continuing to enroll patients through the pandemic and a future analysis will look at those with confirmed COVID-19.
Of the 202 patients in this analysis, 21% had a diagnosis of ARDs, the average age was 50 years old and 61% were women. The researchers collected mucus-based secretions from the trachea (the main airway leading down to the lungs) and extracted DNA to analyze in the laboratory using a technique called next generation sequencing.
Britton said: “We identified about 100 different types of fungi in the lungs of mechanically-ventilated, critically ill patients. The diversity was quite low in all of the samples, but in samples in which a single species dominated, the diversity was very low. A large number of patients had samples that were dominated by a single fungal species from the Candida type; this made up more than 50% of the fungi present in these samples and it is known to be involved in causing disease in humans.”
Fungal diversity was lower in ARDS patients than in those without the disease. In ARDS patients, shock, sepsis and organ failure were associated with lower diversity. Decreased diversity was linked with worse injury to the lungs, more intensive treatment and elevated levels of a protein called pentraxin-3, which is an indicator of inflammation and disease severity.
“The association of lower fungal diversity with clinical markers of disease severity is an important finding because it provides evidence for a relationship between the lung microbiome and clinical outcomes in critical illness,” said Britton. “We will continue to investigate this further because there is much that is not understood about the development and treatment of ARDS and this study may point us towards an important diagnostic or treatment-based discovery.”