Researchers at Brigham and Women’s Hospital have discovered a new vaccine candidate for the bacterium Pseudomonas aeruginosa taking advantage of a new mechanism of immunity. Their findings appear in the American Journal of Respiratory and Critical Care Medicine.
Despite more than 40 years of vaccine research and development, there is no clinically available vaccine for this bacterium. Most prior vaccine efforts have focused on generating antibodies to Pseudomonas toxins or surface molecules, especially the sugar coating on the bug called the lipopolysaccharide O antigen. These approaches have not yielded a licensed vaccine for humans.
For this study, the researchers constructed a vaccine based on a new mechanism of immunity to Pseudomonas mediated by T helper 17 (Th17) cells. Th17 cells are a recently described type of helper T cells that secrete the cytokine IL-17 and enhance antibacterial mucosal defenses.
The investigators designed a screen for Th17-stimulating protein antigens expressed by a molecular library of DNA encoding Pseudomonas proteins. The screen discovered that the Pseudomonas protein PopB is a very effective stimulator of Th17 immunity, and immunization with purified PopB protected mice from lethal pneumonia in an antibody-independent fashion.
The researchers are currently taking their work a step further by constructing conjugate vaccines using PopB as a protein carrier with the hopes of improving the effectiveness of the vaccine. They hope that the PopB-based vaccine might one day be used to prevent Pseudomonas infections in hospitalized patients and in people with cystic fibrosis.
Source: Brigham and Women’s Hospital
